CD279 Monoklonaler Antikörper

CD279 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG1 kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

EH12.2H7

Kat-Nr. : CL750-65168

Synonyme

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2



Geprüfte Anwendungen

Erfolgreiche Detektion in FCPHA treated human PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-65168 bindet in FC CD279 und zeigt Reaktivität mit human, nicht-humane Primaten

Getestete Reaktivität human, nicht-humane Primaten
Wirt / Isotyp Maus / Mouse IgG1 kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name programmed cell death 1
Berechnetes Molekulargewicht 288 aa, 32 kDa
GenBank-ZugangsnummerBC074740
Gene symbol PDCD1
Gene ID (NCBI) 5133
Konjugation CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 750 CD279 antibody CL750-65168Protokoll herunterladen
Standard-Protokolle
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