CD279 Monoklonaler Antikörper

CD279 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG1 kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

EH12.2H7

Kat-Nr. : PE-65168

Synonyme

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood mononuclear cells using PE-65168

  1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with APC Anti-Human CD3 (APC-65151, clone UCHT1) and 10.00 ul/test PE Anti-Human CD279 (PE-65168, Clone:EH12.2H7). Cells were not fixed.

• 

  FC experiment of human peripheral blood mononuclear cells using PE-65168

  1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with APC Anti-Human CD3 (APC-65151, clone UCHT1) and PE mouse IgG1 isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane mononukleäre Zellen aus peripherem Blut Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65168 bindet in FC CD279 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / Mouse IgG1 kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• GenBank-Zugangsnummer: BC074740
• Gene symbol: PDCD1
• Gene ID (NCBI): 5133
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).