CD3e Monoklonaler Antikörper

CD3e Monoklonal Antikörper für FC

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen(4)

CloneNo.

145-2C11

Kat-Nr. : APC-65060

Synonyme

145-2C11, AI504783, CD3, CD3epsilon, CD3ε

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  FC experiment of mouse splenocytes using APC-65060

  1x10^6 mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD3ε (145-2C11) (APC-65060, Clone:145-2C11) or 0.2 ug Isotype Control. Cells were co-stained with PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 4 publications below

Produktinformation

APC-65060 bindet in FC CD3e und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: H-2Kb-spezifischer muriner cytotoxischer T-Lymphozyten (CTL) -Klon BM10-37
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: CD3ε
• Gene ID (NCBI): 12501
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publikationen

Species	Application	Title
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
	FC	Eur J Med Res; Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function.; Authors - Qingqu Guo
mouse	FC	J Adv Res; Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis; Authors - Yi Chen
	FC	J Pharm Biomed Anal; Altered gut metabolites and metabolic reprogramming involved in the pathogenesis of colitis-associated colorectal cancer and the transition of colon "inflammation to cancer"; Authors - Dunfang Wang