CD3ε Monoklonaler Antikörper

CD3ε Monoklonal Antikörper für FC

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC, FC (Intra)

Konjugation

PE Fluorescent Dye

Publikationen(3)

CloneNo.

145-2C11

Kat-Nr. : PE-65060

Synonyme

Cd3e, 145-2C11, AI504783, CD3, CD3epsilon

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  FC experiment of mouse splenocytes using PE-65060

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.10 ug PE Anti-Mouse CD3ε (PE-65060, Clone:145-2C11) (red) or 0.10 ug isotype control antibody (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

PE-65060 bindet in FC, FC (Intra) CD3ε und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: H-2Kb-spezifischer muriner cytotoxischer T-Lymphozyten (CTL) -Klon BM10-37
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: CD3ε
• Gene ID (NCBI): 12501
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and a stabilizer
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publikationen

Species	Application	Title
mouse	FC (Intra)	Adv Healthc Mater; Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization; Authors - Hongping Wan
mouse	FC	Int J Biol Macromol; Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization; Authors - Kai Deng
mouse	FC	J Adv Res; Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis; Authors - Yi Chen