CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

CloneNo.

17A2

Kat-Nr. : CL647-65077

Synonyme

AI504783, CD3, Cd3e, CD3epsilon, T3e

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  IF Staining of mouse splenocytes using CL647-65077

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) at dilution of 1:500.

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  FC experiment of C57BL/6 mouse splenocytes using CL647-65077

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone:17A2) or 0.5 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2) (CL647-65211, Clone: LTF-2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65077 bindet in IF/ICC, FC CD3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: γδTCR-positive T-T hybridoma D1
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: CD3ε
• Gene ID (NCBI): 12501
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publikationen

Species	Application	Title
mouse		Biomed Pharmacother; IDO1 inhibitors are synergistic with CXCL10 agonists in inhibiting colon cancer growth; Authors - Mengdi Yang