CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

390

Kat-Nr. : 65058-1-Ig

Synonyme

390, Pecam, PECAM 1, Pecam1, PECAM-1

---

• 

  IF Staining of bEnd.3 using 65058-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (65058-1-Ig, Clone: 390 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rat IgG(H+L).

• 

  FC experiment of mouse splenocytes using 65058-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD31 (65058-1-Ig, Clone: 390) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100 (blue). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: bEnd.3-Zellen
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 8 publications below

Produktinformation

65058-1-Ig bindet in IF/ICC, FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• GenBank-Zugangsnummer: BC008519
• Gene symbol: CD31
• Gene ID (NCBI): 18613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publikationen

Species	Application	Title
	IF	mBio; Human ApoE2 protects mice against Plasmodium berghei ANKA experimental cerebral malaria; Authors - Rui Liang
mouse	IF	J Ethnopharmacol; Investigation of the efficacy of Dengzhan Shengmai capsule against heart failure with preserved ejection fraction; Authors - Ziyi Kang
mouse	IF	Neuropharmacology; Δ9-tetrahydrocannabinol induces blood-brain barrier disruption: Involving the activation of CB1R and oxidative stress; Authors - Qianyao Zhang
mouse	IF	J Ethnopharmacol; Berberis dictyophylla F. inhibits angiogenesis and apoptosis of diabetic retinopathy via suppressing HIF-1α/VEGF/DLL-4/Notch-1 pathway.; Authors - Xiaopeng Ai
mouse	IF	Eur J Pharmacol; Crosstalk between endothelial cells with a non-canonical EndoMT phenotype and cardiomyocytes/fibroblasts via IGFBP5 aggravates TAC-induced cardiac dysfunction; Authors - Yue Li
		Phytother Res; A new look at angiogenesis inhibition of geniposide in experimental arthritis by blocking angiopoietin-2 exocytosis; Authors - Peirong Gan