CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

CloneNo.

390

Kat-Nr. : CL647-65058

Synonyme

390, Pecam, PECAM 1, Pecam1, PECAM-1

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  IF Staining of bEnd.3 using CL647-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 647 CD31 antibody (CL647-65058, Clone: 390) at dilution of 1:100.

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  FC experiment of C57BL/6 mouse bone marrow cells using CL647-65058

  1x10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse CD31 (CL647-65058, Clone:390), and 0.2 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3), and 0.2 ug FITC Plus Anti-Mouse CD45R (B220) (RA3-6B2) (FITC-65139, Clone: RA3-6B2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: bEnd.3-Zellen
• Erfolgreiche Detektion in FC: C57BL/6 mouse bone marrow cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL647-65058 bindet in IF/ICC, FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• GenBank-Zugangsnummer: BC008519
• Gene symbol: CD31
• Gene ID (NCBI): 18613
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publikationen

Species	Application	Title
	IF	iScience; SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway; Authors - Kai Yang