CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus und mehr (1)

Anwendung

IF, FC

Konjugation

FITC Fluorescent Dye

Publikationen

3

CloneNo.

390

Kat-Nr. : FITC-65058

Synonyme

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Galerie der Validierungsdaten

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  IF Staining of bEnd.3 using FITC-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) at dilution of 1:100.

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  FC experiment of mouse splenocytes using FITC-65058

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD31 (FITC-65058, Clone:390) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: bEnd.3-Zellen
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 2 publications below

Produktinformation

FITC-65058 bindet in IF, FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• GenBank-Zugangsnummer: BC008519
• Gene symbol: Pecam1
• Gene ID (NCBI): 18613
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publikationen

Species	Application	Title
mouse	IF	Cell Death Dis; LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease.; Authors - Hong Su
mouse	FC	Bone; mTORC1 coordinates NF-κB signaling pathway to promote chondrogenic differentiation of tendon cells in heterotopic ossification; Authors - Jiaming Fu
rat	FC	Cells; Enhancement of Tendon Repair Using Tendon-Derived Stem Cells in Small Intestinal Submucosa via M2 Macrophage Polarization; Authors - Xufeng Mao