CD4 Polyklonaler Antikörper

CD4 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 11056-2-AP

Synonyme

CD4, CD4 molecule, CD4mut

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• 

  WB analysis of human spleen using 11056-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11056-2-AP (CD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen tissue using 11056-2-AP

  IP result of anti-CD4 (11056-2-AP for IP and Detection) with mouse spleen tissue lysate.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe
• Erfolgreiche IP: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Produktinformation

11056-2-AP bindet in WB, IP, FC, ELISA CD4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD4 fusion protein Ag0422
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Publikationen

Species	Application	Title
mouse		ACS Appl Mater Interfaces; NIR-Triggered Sequentially Responsive Nanocarriers Amplified Cascade Synergistic Effect of Chemo-Photodynamic Therapy with Inspired Antitumor Immunity.; Authors - Feiyang Jin
human		Cancer Lett; A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity.; Authors - Huimin Tao
human	WB	Front Immunol; PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation; Authors - Rui Su
mouse		EBioMedicine; Enho Mutations Causing Low Adropin: A Possible Pathomechanism of MPO-ANCA Associated Lung Injury.; Authors - Feng Gao
		Am J Cancer Res; Changes in the intestinal microecology induced by bacillus subtilis inhibit the occurrence of ulcerative colitis and associated cancers: a study on the mechanisms.; Authors - Chuancong Wu
mouse		J Transl Med; Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model.; Authors - Han Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Luca (Verified Customer) (02-26-2018)

• Applications: Other (please state below),
• Primary Antibody Dilution: 10000 to 1 ng/ml