CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen(2)

CloneNo.

RM4-5

Kat-Nr. : APC-65141

Synonyme

CD4 antigen, L3T4, Ly 4, RM4-5, T-cell differentiation antigen L3T4

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  FC experiment of mouse splenocytes using APC-65141

  1X10^6 mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD4 (APC-65141, Clone:RM4-5) or 0.1 ug APC Rat IgG2a Isotype Control (2A3) (APC-65209, Clone: 2A3), and 0.1 ug PE Anti-Mouse CD3 (17A2) (PE-65077, Clone: 17A2). Cells were not fixed.

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  FC experiment of mouse splenocytes using APC-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug APC-anti-mouse CD4 (APC-65141, clone RM4-5) (red) or 0.20 ug APC-rat IgG2a isotype control (blue). Cells were not fixed. .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

APC-65141 bindet in FC CD4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BALB/c Maus-Thymozyten
• Vollständiger Name: CD4 antigen
• GenBank-Zugangsnummer: BC039137
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publikationen

Species	Application	Title
		Nat Commun; In situ valence-transited arsenic nanosheets for multi-modal therapy of colorectal cancer; Authors - Hongyue Zheng
mouse	FC	Adv Healthc Mater; Bimetallic MnZnSX Nanotheranostics for Self-Activatable Chemo-Immunotherapy of Hepatocellular Carcinoma via H₂S-Triggered Arsenic Prodrug Activation and Binary cGAS-STING Pathway Modulation; Authors - WeiYi Cheng