CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen

3

CloneNo.

GK1.5

Kat-Nr. : FITC-65104

Synonyme

Cd4, CD4 antigen, L3T4, Ly 4

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using FITC-65104

  1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug FITC Plus Anti-Mouse CD4 (FITC-65104, Clone: GK1.5) or 0.5 ug FITC Plus rat IgG2b isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 3 publications below

Produktinformation

FITC-65104 bindet in FC CD4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-CTL-Klon-V4
• Vollständiger Name: CD4 antigen
• GenBank-Zugangsnummer: BC039137
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publikationen

Species	Application	Title
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC	J Mater Chem B; PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer; Authors - Lian Zhu
mouse	FC	Stem Cells Int; Oral Cancer Stem Cell-Derived Small Extracellular Vesicles Promote M2 Macrophage Polarization and Suppress CD4+ T-Cell Activity by Transferring UCA1 and Targeting LAMC2; Authors - Lan Wu