CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

FC, FC (Intra)

Konjugation

PE Fluorescent Dye

Publikationen(1)

CloneNo.

OKT4

Kat-Nr. : PE-65134

Synonyme

CD4 molecule, CD4mut, OKT4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

---

• 

  FC experiment of human PBMCs using PE-65134

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD4 (OKT4) (PE-65134, Clone: OKT4) or PE Mouse IgG2b Isotype Control (MPC-11) (PE-65128, Clone: MPC-11), and 5 ul CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-65134

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD4 (OKT4) (PE-65134, Clone:OKT4) or PE Mouse IgG2b Isotype Control (MPC-11) (PE-65128, Clone: MPC-11). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65134 bindet in FC, FC (Intra) CD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane periphere T-Zellen
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.1% gelatin
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publikationen

Species	Application	Title
human	FC (Intra)	Cardiovasc Res; Senescent CD8+ T cells: a novel risk factor in atrial fibrillation; Authors - Xiang Li