CD42b Monoklonaler Antikörper

CD42b Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

AK2

Kat-Nr. : CL405-65163

Synonyme

BSS, CD42B, CD42b alpha, GP1B, GP1BA



Geprüfte Anwendungen

Erfolgreiche Detektion in FChuman peripheral blood platelets

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65163 bindet in FC CD42b und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Humane Thrombozyten
Vollständiger Name glycoprotein Ib (platelet), alpha polypeptide
Berechnetes Molekulargewicht 626 aa, 69 kDa
GenBank-ZugangsnummerBC027955
Gene symbol GP1BA
Gene ID (NCBI) 2811
Konjugation CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Reinigungsmethode Mit Protein-A-Affinitätschromatographie gereinigt
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 405 CD42b antibody CL405-65163Protokoll herunterladen
Standard-Protokolle
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