CD69 Monoklonaler Antikörper

CD69 Monoklonal Antikörper für FC

Wirt / Isotyp

Armenian Hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(3)

CloneNo.

H1.2F3

Kat-Nr. : FITC-65105

Synonyme

VEA, H1.2F3, CD69 antigen, AIM

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  FC experiment of mouse splenocytes using FITC-65105

  1x10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were surface stained with 0.5 ug FITC Plus Anti-Mouse CD69 (H1.2F3) (FITC-65105, Clone: H1.2F3) and CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 3 publications below

Produktinformation

FITC-65105 bindet in FC CD69 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Armenian Hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus dendritische epidermale T-Zelllinie Y245
• Vollständiger Name: CD69 antigen
• GenBank-Zugangsnummer: BC106997
• Gene symbol: Cd69
• Gene ID (NCBI): 12515
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.1% gelatin
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Publikationen

Species	Application	Title
mouse	FC	Nat Nanotechnol; A bispecific nanosystem activates endogenous natural killer cells in the bone marrow for haematologic malignancies therapy; Authors - Yanqin Zhang
mouse	FC	Adv Healthc Mater; Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization; Authors - Hongping Wan
mouse	FC	Int J Biol Macromol; Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization; Authors - Kai Deng