CD71 Monoklonaler Antikörper

CD71 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OKT9

Kat-Nr. : CL647-65235

Synonyme

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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  FC experiment of human PBMCs using CL647-65235

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 (CL647-65235, Clone: OKT9), or APC Mouse IgG1 Isotype Control. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-65235

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 (CL647-65235, Clone: OKT9), or APC Mouse IgG1 Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65235 bindet in FC CD71 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.