CD73 Monoklonaler Antikörper

CD73 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG1 kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

AD2

Kat-Nr. : PE-65162

Synonyme

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), AD2, CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood lymphocytes using PE-65162

  1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD3 (APC-65148, clone SK7) and 10.00 ul/test PE-Anti-Human CD73 (PE-65162, Clone:AD2). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using PE-65162

  1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD3 (APC-65148, clone SK7) and PE-mouse IgG1 isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65162 bindet in FC CD73 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / Mouse IgG1 kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 29 kDa, 63 kDa
• GenBank-Zugangsnummer: BC015940
• Gene symbol: NT5E
• Gene ID (NCBI): 4907
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).