CD8a Monoklonaler Antikörper

CD8a Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

APC Fluorescent Dye

Publikationen(15)

CloneNo.

53-6.7

Kat-Nr. : APC-65069

Synonyme

CD8, 53-6.7, Ly 35, Ly 2, CD8α

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  FC experiment of mouse splenocytes using APC-65069

  1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug APC Anti-Mouse CD8a (APC-65069, Clone:53-6.7) or 0.2 ug Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 11 publications below

Produktinformation

APC-65069 bindet in IF, FC CD8a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Milzzellen oder Thymozytenmembranen
• Vollständiger Name: CD8 antigen, alpha chain
• GenBank-Zugangsnummer: BC030679
• Gene symbol: Cd8a
• Gene ID (NCBI): 12525
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publikationen

Species	Application	Title
mouse	FC	Drug Deliv; Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes.; Authors - Dingyi Wang
mouse	FC	Front Pharmacol; In-situ formed thermosensitive hydrogel amplifies statin-mediated immune checkpoint blockade for coordinated tumor chemo-immunotherapy; Authors - Zefan Liu
mouse	FC	Vet Microbiol; A self-assembled nanoparticle vaccine based on pseudorabies virus glycoprotein D induces potent protective immunity against pseudorabies virus infection; Authors - Xujiao Ren
mouse	FC	Animals (Basel); An Intracellular Epitope of ASFV CD2v Protein Elicits Humoral and Cellular Immune Responses; Authors - Wenlong Lu
mouse	IF	J Med Chem; Platinum(IV) Complexes as Inhibitors of STAT3 and Regulators of the Tumor Microenvironment To Control Breast Cancer; Authors - Linxiang Cai
mouse	FC	J Nanobiotechnology; The combination of modified acupuncture needle and melittin hydrogel as a novel therapeutic approach for rheumatoid arthritis treatment; Authors - Lisha Liu