CD80 Monoklonaler Antikörper

CD80 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2D10.4

Kat-Nr. : 65083-1-Ig

Synonyme

2D10.4, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1

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  FC experiment of Daudi using 65083-1-Ig

  1x10^6 Daudi cells were surface stained with 0.2 ug Anti-Human CD80 (65083-1-Ig, Clone: 2D10.4) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

• 

  FC experiment of Daudi using 65083-1-Ig

  1x10^6 Daudi cells were surface stained with 0.2 ug Anti-Human CD80 (65083-1-Ig, Clone: 2D10.4) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Daudi-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

65083-1-Ig bindet in FC CD80 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD80 molecule
• Berechnetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC042665
• Gene symbol: CD80
• Gene ID (NCBI): 941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publikationen

Species	Application	Title
human	FC	Proc Natl Acad Sci U S A; Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell function; Authors - Hideki Hayashi