CD86 Rekombinanter Antikörper

CD86 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230476A1

Kat-Nr. : 83213-5-RR

Synonyme

230476A1, B7, B7 2, B7.2, B70

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  WB analysis using 83213-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83213-5-RR (CD86 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83213-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83213-5-RR against Mouse CD86 were performed. The affinity constant is 97.9 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DC2.4-Zellen, J774A.1 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83213-5-RR bindet in WB, ELISA CD86 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD86 fusion protein Eg0802
• Vollständiger Name: CD86 antigen
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: NM_019388
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)