CD90.2 Monoklonaler Antikörper

CD90.2 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

30-H12

Kat-Nr. : PE-65088

Synonyme

CD90, Thy1, 30-H12, Thy 1, Thy-1

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  FC experiment of mouse thymocytes using PE-65088

  1x10^6 mouse thymocytes were surface stained with 0.1 ug PE Anti-Mouse CD90.2 (30-H12) (PE-65088, Clone:30-H12) (red), or 0.1 ug PE Rat IgG2b Isotype Control (LTF-2) (PE-65211, Clone: LTF-2) (blue). Cells were not fixed.

• 

  FC experiment of mouse thymocytes using PE-65088

  1x10^6 mouse thymocytes were surface stained with 0.1 ug PE Anti-Mouse CD90.2 (30-H12) (PE-65088, Clone:30-H12), or 0.1 ug PE Rat IgG2b Isotype Control (LTF-2) (PE-65211, Clone: LTF-2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Thymozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65088 bindet in FC CD90.2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-Thymus oder -milz
• Vollständiger Name: thymus cell antigen 1, theta
• GenBank-Zugangsnummer: BC054436
• Gene symbol: CD90
• Gene ID (NCBI): 21838
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).