DKK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DKK2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 21051-1-AP

Synonyme

Dickkopf 2, Dickkopf related protein 2, DKK 2, DKK2, hDkk 2

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 21051-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21051-1-AP (DKK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21051-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21051-1-AP (DKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 21051-1-AP

  IP Result of anti-DKK2 (IP:21051-1-AP, 4ug; Detection:21051-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human cervix using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixgewebe, humanes Hautkrebsgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

21051-1-AP bindet in WB, IP, IHC, IF, ELISA DKK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DKK2 fusion protein Ag12405
• Vollständiger Name: dickkopf homolog 2 (Xenopus laevis)
• Berechnetes Molekulargewicht: 259 aa, 28 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC075078
• Gene symbol: DKK2
• Gene ID (NCBI): 27123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Mol Neurobiol; Ionizing Radiation Blocks Hair Cell Regeneration in Zebrafish Lateral Line Neuromasts by Preventing Wnt Signaling.; Authors - Rong Li
mouse	WB	Int J Mol Sci; Roles of Dkk2 in the Linkage from Muscle to Bone during Mechanical Unloading in Mice.; Authors - Naoyuki Kawao
human	IHC	Front Endocrinol (Lausanne); G Protein-Coupled Estrogen Receptor Correlates With Dkk2 Expression and Has Prognostic Impact in Ovarian Cancer Patients.; Authors - Patricia Fraungruber
mouse	WB	Exp Clin Endocrinol Diabetes; Role of Dkk2 in the Muscle/bone Interaction of Androgen-Deficient Mice; Authors - Shunki Iemura
mouse	WB	Cell Death Discov; Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow.; Authors - Fengchan Li
human	WB,IHC	J Immunol Res; DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma.; Authors - Jianyu Yang ; KD Validated