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Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293, TF-1, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87728-1-RR targets Darc in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Duffy blood group, chemokine receptor |
| Observed Molecular Weight | 38 kDa, 43 kDa |
| GenBank Accession Number | BC005583 |
| Gene Symbol | Darc |
| Gene ID (NCBI) | 13349 |
| RRID | AB_3745721 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9QUI6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse Darc (Ackr1) is an atypical chemokine receptor expressed predominantly on erythrocytes and endothelial cells, where it binds diverse inflammatory chemokines without classical GPCR signaling. By functioning as a chemokine reservoir and transporter, Ackr1 regulates chemokine gradients and leukocyte trafficking during inflammation. Studies in Ackr1-deficient mice highlight its role in immune homeostasis and neutrophil recruitment, establishing Darc as an important regulator of systemic chemokine availability and inflammatory responses.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Darc antibody 87728-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
