E2F3 Polyklonaler Antikörper

E2F3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12334-1-AP

Synonyme

E2F3, Q96AR0

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Galerie der Validierungsdaten

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12334-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12334-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12334-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12334-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse liver using 12334-1-AP

  IP Result of anti-E2F3 (IP:12334-1-AP, 4ug; Detection:12334-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HEK-293-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

12334-1-AP bindet in WB, IP, IHC, ELISA E2F3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: E2F3 fusion protein Ag3039
• Vollständiger Name: E2F transcription factor 3
• Berechnetes Molekulargewicht: 133 aa, 15 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC016847
• Gene symbol: E2F3
• Gene ID (NCBI): 1871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E2F transcription factors regulate gene expression in concert with the retinoblastoma tumor suppressor family. These transcriptional complexes are master regulators of cell cycle progression, control the expression of genes involved in DNA repair, G2/M checkpoint and differentiation [PMID:22580460]. E2f3 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner [PMID:22960857].

Publikationen

Species	Application	Title
human	WB, IHC	Nat Commun; POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.; Authors - Boshi Wang
human	WB	Mol Med Rep; [Corrigendum] Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.; Authors - Dacai Xu
human	WB	Mol Med Rep; Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.; Authors - Dacai Xu