Fibromodulin Monoklonaler Antikörper

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• KD/KO Validated

Fibromodulin Monoklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

CloneNo.

3E9D10

Kat-Nr. : 60108-1-Ig

Synonyme

fibromodulin, FM, FMOD, KSPG fibromodulin, SLRR2E

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  WB analysis of human kidney using 60108-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 60108-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 60108-1-Ig

  human stomach tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 60108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 60108-1-Ig (Fibromodulin antibody) at dilution of 1:300 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 60108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 60108-1-Ig (Fibromodulin antibody) at dilution of 1:300 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Produktinformation

60108-1-Ig bindet in WB, IHC, IF, ELISA Fibromodulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fibromodulin fusion protein Ag4030
• Vollständiger Name: fibromodulin
• Berechnetes Molekulargewicht: 376 aa, 43 kDa
• GenBank-Zugangsnummer: BC035281
• Gene symbol: Fibromodulin
• Gene ID (NCBI): 2331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).

Publikationen

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution; Authors - Taifeng Zhou
mouse	IHC	J Clin Invest; Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment.; Authors - Irit Adini ; KD Validated
mouse	IF	Acta Biomater; In situ regeneration of bone-to-tendon structures: Comparisons between costal-cartilage derived stem cells and BMSCs in the rat model.; Authors - Rui Zuo
human		FASEB J; Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential.; Authors - Irit Adini
human,mouse	WB,IF	BMC Cancer; Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis; Authors - Yufei Wang
rat	WB	Cartilage; Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel; Authors - Qiang Chai