Fibulin 5 Monoclonal antibody

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• Fibulin 5 Monoclonal antibody (60081-1-Ig)

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Fibulin 5 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 60081-1-Ig Copied!

Clone No. 2A5C8

Publications (7)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

FBLN5, 2A5C8, Dance, Developmental arteries and neural crest EGF-like protein, EVEC

• 

  WB analysis of MCF-7 using 60081-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 60081-1-Ig

  WB result of Fibulin 5 antibody (60081-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibulin 5 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of pig heart using 60081-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 60081-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 60081-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 60081-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 60081-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 60081-1-Ig(Fibulin 5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, pig heart tissue, human placenta tissue, human heart tissue, rat heart tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60081-1-Ig targets Fibulin 5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2859

  Product name: Recombinant human FBLN5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 186-448 aa of BC022280

  Sequence: GSYSCTCNPGFTLNEDGRSCQDVNECATENPCVQTCVNTYGSFICRCDPGYELEEDGVHCSDMDECSFSEFLCQHECVNQPGTYFCSCPPGYILLDDNRSCQDINECEHRNHTCNLQQTCYNLQGGFKCIDPIRCEEPYLRISDNRCMCPAENPGCRDQPFTILYRDMDVVSGRSVPADIFQMQATTRYPGAYYIFQIKSGNEGREFYMRQTGPISATLVMTRPIKGPREIQLDLEMITVNTVINFRGSSVIRLRIYVSQYPF

• Full Name: fibulin 5
• Calculated Molecular Weight: 448 aa, 50 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC022280
• Gene Symbol: Fibulin 5
• Gene ID (NCBI): 10516
• RRID: AB_2231920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UBX5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.

Publications

What published studies show

The Fibulin 5 Monoclonal antibody (2A5C8), catalog number 60081-1-Ig, has 7 total citations. These appear in journals including Advanced Healthcare Materials, Free Radical Biology and Medicine, Matrix Biology, Materials Science and Engineering C, Stem Cells International, PLoS One, and Journal of Biomolecular Screening. Associated research fields span pelvic organ prolapse treatment, vascular smooth muscle TNFα signaling, ovarian proteomics and fertility biomarkers, bone repair and inflammation, IgG4-related lacrimal gland fibrosis, nasopharyngeal carcinoma metastasis, and fibulin-3 secretion regulation.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Adv Healthc Mater; Galvanic Cell-Stimulated Mesenchymal Stem Cell Mesh for Enhanced Pelvic Organ Prolapse Treatment; Authors - Ao Xiao
mouse	WB,IP	Free Radic Biol Med; Extracellular SOD modulates canonical TNFα signaling and α5β1 integrin transactivation in vascular smooth muscle cells; Authors - Hyehun Choi ; KD Validated
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
mouse	WB,IF	Mater Sci Eng C Mater Biol Appl; Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation.; Authors - Dongdong Yao
human	WB	Stem Cells Int; Effects of Fibulin-5 Gene Silencing on Proliferation and Apoptosis of IgG4-ROD Lacrimal Gland Fibroblasts; Authors - Huarong Wu
human	IHC	PLoS One; Oncogenic Fibulin-5 Promotes Nasopharyngeal Carcinoma Cell Metastasis through the FLJ10540/AKT Pathway and Correlates with Poor Prognosis.; Authors - Chung-Feng Hwang