GABRB1 Polyklonaler Antikörper

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• KD/KO Validated

GABRB1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 20183-1-AP

Synonyme

GABA A receptor beta 1, GABA A receptor β, GABAA receptor beta 1, GABRB1

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  WB analysis of mouse testis using 20183-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20183-1-AP (GABRB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20183-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20183-1-AP (GABRB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 20183-1-AP

  IP result of anti-GABRB1 (IP:20183-1-AP, 4ug; Detection:20183-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

20183-1-AP bindet in WB, IP, IF, ELISA GABRB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABRB1 fusion protein Ag14070
• Vollständiger Name: gamma-aminobutyric acid (GABA) A receptor, beta 1
• Berechnetes Molekulargewicht: 474 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-54 kDa
• GenBank-Zugangsnummer: BC022449
• Gene symbol: GABRB1
• Gene ID (NCBI): 2560
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse	WB	iScience; Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1-/- mice; Authors - Jing Wang ; KO Validated
mouse	WB	Food Funct; Ginsenoside Rg5/Rk1 ameliorated sleep via regulating the GABAergic/serotoninergic signaling pathway in a rodent model.; Authors - Jingjing Shao
human	WB,IF	Reprod Fertil Dev; Sperm gamma-aminobutyric acid type A receptor delta subunit (GABRD) and its interaction with purinergic P2X2 receptors in progesterone-induced acrosome reaction and male fertility.; Authors - Wenming Xu
	WB	Mol Nutr Food Res; As a Histone Deacetylase Inhibitor, γ-Aminobutyric Acid Upregulates GluR2 Expression: An In Vitro and In Vivo Study.; Authors - Aiping Xing