GBX2 Polyklonaler Antikörper

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• KD/KO Validated

GBX2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21639-1-AP

Synonyme

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2

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  WB analysis of HeLa using 21639-1-AP

  WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells.

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  WB analysis of mouse stomach using 21639-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21639-1-AP

  IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IF Staining of HeLa using 21639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (21639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Magengewebe, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• ChIP: See 2 publications below

Produktinformation

21639-1-AP bindet in WB, IHC, IF/ICC, IP, chIP, ELISA GBX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GBX2 fusion protein Ag16288
• Vollständiger Name: gastrulation brain homeobox 2
• Berechnetes Molekulargewicht: 348 aa, 37 kDa
• Beobachtetes Molekulargewicht: 33-37 kDa
• GenBank-Zugangsnummer: BC137448
• Gene symbol: GBX2
• Gene ID (NCBI): 2637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

Publikationen

Species	Application	Title
human	IF	Cell Stem Cell; Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders; Authors - David Shin
human	chIP	RNA Biol; NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.; Authors - Xiwen Li
human	WB,ChIP	Funct Integr Genomics; Oncogenic GBX2 promotes the malignant behaviors of bladder cancer cells by binding to the ITGA5 promoter and activating its transcription.; Authors - Yaoyao Xiong
human	IF	Life Med; CRISPR/Cas9-mediated genetic correction reverses spinocerebellar ataxia 3 disease-associated phenotypes in differentiated cerebellar neurons; Authors - Guoxu Song
human	IHC,IF	Front Neuroanat; Development of the early fetal human thalamus: from a protomap to emergent thalamic nuclei; Authors - Maznah Alhesain