HCN2 Polyklonaler Antikörper

HCN2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 55245-1-AP

Synonyme

HAC 1, HCN2

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55245-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55245-1-AP (HCN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55245-1-AP (HCN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55245-1-AP

  IP Result of anti-HCN2 (IP:55245-1-AP, 4ug; Detection:55245-1-AP 1:800) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human heart using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Jurkat-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

55245-1-AP bindet in WB, IP, IHC, IF, ELISA HCN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97-110 kDa
• GenBank-Zugangsnummer: NM_001194
• Gene symbol: HCN2
• Gene ID (NCBI): 610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). HCN2 produces a large instantaneous current. It is activated by cAMP. HCN2 is modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. This antibody is specific to HCN2.

Publikationen

Species	Application	Title
human	WB,IF	Nucleic Acids Res; Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts.; Authors - Donghong Zhang
rat	WB	Mol Pain; HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons.; Authors - Xiaoyu Liu
rat	WB,IHC	Brain Res Bull; HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling.; Authors - Xiaoyu Liu
mouse	WB	Exp Ther Med; Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.; Authors - Chandong Ding
mouse	WB	Cell Rep; Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.; Authors - Giuseppe Sciamanna
mouse	WB	Neurosci Bull; Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus; Authors - Yi Yan ; KD Validated