Histone H1.2 Polyklonaler Antikörper

Histone H1.2 Polyklonal Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15446-1-AP

Synonyme

H1.2, H1F2, HIST1H1C, histone, histone cluster 1, H1c, Histone H1.2, Histone H1d

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  WB analysis of Jurkat using 15446-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15446-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15446-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15446-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 15446-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15446-1-AP (Histone H1.2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hodengewebe, L02-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

15446-1-AP bindet in WB, IF, ELISA Histone H1.2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H1.2 fusion protein Ag7607
• Vollständiger Name: histone cluster 1, H1c
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 32-33 kDa
• GenBank-Zugangsnummer: BC002649
• Gene symbol: Histone H1.2
• Gene ID (NCBI): 3006
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Immunity; The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.; Authors - Dapeng Wang
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	WB	Nat Commun; Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.; Authors - Chen Xu
human	WB	Cell Physiol Biochem; Tumor-Secreted Exosomal miR-222 Promotes Tumor Progression via Regulating P27 Expression and Re-Localization in Pancreatic Cancer.; Authors - Zhonghu Li
human	WB	Stem Cell Res Ther; Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.; Authors - Ruijie Zhang
human	WB	Oxid Med Cell Longev; Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels.; Authors - Reiko Iida