HTR3A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HTR3A Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10443-1-AP

Synonyme

5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 10443-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10443-1-AP (HTR3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10443-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10443-1-AP (HTR3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of SH-SY5Y using 10443-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug HTR3A antibody (10443-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10443-1-AP bindet in WB, IHC, IF, FC, ELISA HTR3A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HTR3A fusion protein Ag0289
• Vollständiger Name: 5-hydroxytryptamine (serotonin) receptor 3A
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC002354
• Gene symbol: HTR3A
• Gene ID (NCBI): 3359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)

Publikationen

Species	Application	Title
human	WB	Pain; Sex differences in serotonin enhancement of capsaicin-evoked calcitonin gene-related peptide release from human dental pulp.; Authors - Loyd Dayna R DR
rat	WB, IHC	Front Pharmacol; Involvement of 5-Serotonin and Substance p Pathways in Dichroa Alkali Salt-Induced Acute Pica in Rats.; Authors - Lina Ma
rat	IF	Sci Rep; Oral Phyto-thymol ameliorates the stress induced IBS symptoms.; Authors - Selvaraj Subramaniyam
mouse	WB	Front Endocrinol (Lausanne); Electroacupuncture Attenuates Post-Inflammatory IBS-Associated Visceral and Somatic Hypersensitivity and Correlates With the Regulatory Mechanism of Epac1-Piezo2 Axis.; Authors - Jing Guo
mouse	WB	Biochem Biophys Res Commun; FSH signaling is involved in affective disorders.; Authors - Wen-Kai Bi
human	WB	Oncol Lett; Downregulation of 5-hydroxytryptamine receptor 3A expression exerts an anticancer activity against cell growth in colorectal carcinoma cells in vitro.; Authors - Jian Tang ; KD Validated