IL-7 Rekombinanter Antikörper

IL-7 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241000F3

Kat-Nr. : 98206-1-PBS

Synonyme

IL7, Interleukin-7, interleukin 7, IL 7, 241000F3

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  FC experiment of Transfected HEK-293T using 98206-1-RR (same clone as 98206-1-PBS)

  1x10^6 HEK-293T cells or IL-7 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98206-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98206-1-RR (same clone as 98206-1-PBS)

  1x10^6 HEK-293T cells (left) or IL-7 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98206-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98206-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98206-1-RR against Human IL-7 were performed. The affinity constant is 8.55 nM.

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Geprüfte Anwendungen

Produktinformation

98206-1-PBS bindet in FC (Intra) IL-7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 7
• Berechnetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: NM_000880.4
• Gene symbol: IL-7
• Gene ID (NCBI): 3574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)