LAG-3/CD223 Rekombinanter Antikörper

LAG-3/CD223 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

243086D4

Kat-Nr. : 98435-2-RR

Synonyme

CD223, LAG3, FDC, LAG 3, LAG-3

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  FC experiment of human PBMCs using 98435-2-RR

  1x10^6 untreated or anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 0.25 ug Anti-Human LAG-3/CD223 Rabbit RecAb (98435-2-RR, Clone: 243086D4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (CL647-65559, Clone: UCHT4). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98435-2-RR

  1x10^6 anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 0.25 ug Anti-Human LAG3 Rabbit RecAb (98435-2-RR, Clone: 243086D4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human LAG3 Rabbit RecAb (98435-2-RR, Clone: 243086D4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98435-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98435-2-RR against Human LAG-3/CD223 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 and IL-2 treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98435-2-RR bindet in FC LAG-3/CD223 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LAG-3/CD223 fusion protein Eg31550
• Vollständiger Name: lymphocyte-activation gene 3
• Berechnetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: NM_002286
• Gene symbol: LAG-3
• Gene ID (NCBI): 3902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)