EDG2/LPA1 Polyklonaler Antikörper

EDG2/LPA1 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20442-1-AP

Synonyme

LPAR1, edg 2, EDG2, Gpcr26, GPR26

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  WB analysis of Jurkat using 20442-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 20442-1-AP

  Multi-cells/tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20442-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 20442-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 20442-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug EDG2 Polyclonal antibody (20442-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A2780-Zellen, A375-Zellen, A549-Zellen, HeLa-Zellen, Maushirngewebe, Multizellen/-gewebe, NIH3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

20442-1-AP bindet in WB, IHC, FC (Intra), ELISA EDG2/LPA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EDG2/LPA1 fusion protein Ag14253
• Vollständiger Name: lysophosphatidic acid receptor 1
• Berechnetes Molekulargewicht: 364 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41-50 kDa
• GenBank-Zugangsnummer: BC030615
• Gene symbol: EDG2
• Gene ID (NCBI): 1902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EDG2 (also known as LPA1) is a G-protein coupled receptor for lysophosphatidic acid (LPA), a potent motility inducing factor, which is a major component of serum (PMID: 19415462). EDG2 is widely expressed in normal tissue during growth and development. In the context of cancer, several studies have suggested that EDG2 expression in tumors is often similar to that shown in normal tissue (PMID: 17496233).

Publikationen

Species	Application	Title
human	WB	Mediators Inflamm; AM966, an Antagonist of Lysophosphatidic Acid Receptor 1, Increases Lung Microvascular Endothelial Permeability through Activation of Rho Signaling Pathway and Phosphorylation of VE-Cadherin.; Authors - Junting Cai
mouse,human	WB,IHC	Pediatr Res; Bronchopulmonary dysplasia demonstrates dysregulated autotaxin/lysophosphatidic acid signaling in a neonatal mouse model; Authors - Alison W Ha
human	WB	Cell Death Dis; S1PR3-driven positive feedback loop sustains STAT3 activation and keratinocyte hyperproliferation in psoriasis; Authors - Panpan Lian
mouse	WB	J Control Release; Mannose-modified exosomes loaded with MiR-23b-3p target alveolar macrophages to alleviate acute lung injury in Sepsis; Authors - Jiezhi Lin