MED12L Polyklonaler Antikörper

MED12L Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20047-1-AP

Synonyme

KIAA1635, Mediator complex subunit 12-like protein, Mediator of RNA polymerase II transcription subunit 12-like protein, PRO0314, Thyroid hormone receptor-associated-like protein

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  WB analysis of mouse liver using 20047-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20047-1-AP (MED12L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 20047-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MED12L antibody (20047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20047-1-AP bindet in WB, IF/ICC, ELISA MED12L und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mediator complex subunit 12-like
• Berechnetes Molekulargewicht: 240 kDa
• Beobachtetes Molekulargewicht: 260-280 kDa
• GenBank-Zugangsnummer: NM_053002
• Gene symbol: MED12L
• Gene ID (NCBI): 116931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The antibody can recognize all the isoform of MED12L. The antibody has no cross reaction to MED12.