MHC Class II (I-A/I-E) Monoklonaler Antikörper

MHC Class II (I-A/I-E) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

M5/114.15.2

Kat-Nr. : CL405-65122

Synonyme

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II



Geprüfte Anwendungen

Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65122 bindet in FC MHC Class II (I-A/I-E) und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Ratte / IgG2b, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Aktivierte C57BL/6-Mausmilzzellen
Vollständiger Name histocompatibility 2, class II antigen A, alpha
GenBank-ZugangsnummerBC031711
Gene symbol H2-Aa
Gene ID (NCBI) 14960
Konjugation CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 405 MHC Class II (I-A/I-E) antibody CL405-65122Protokoll herunterladen
Standard-Protokolle
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