MOGAT2 Polyklonaler Antikörper

MOGAT2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19514-1-AP

Synonyme

DC5, DGAT2L5, hDC5, hMGAT2, MGAT2, MOGAT2

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Galerie der Validierungsdaten

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  WB analysis using 19514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19514-1-AP (MOGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19514-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19514-1-AP (MOGAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19514-1-AP

  IP Result of anti-MOGAT2 (IP:19514-1-AP, 4ug; Detection:19514-1-AP 1:300) with HEK-293 cells lysate 4800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

19514-1-AP bindet in WB, IP, ELISA MOGAT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: monoacylglycerol O-acyltransferase 2
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: NM_025098
• Gene symbol: MOGAT2
• Gene ID (NCBI): 80168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MOGAT2, also named as DC5 and DGAT2L5, belongs to the diacylglycerol acyltransferase family. MOGAT2 catalyze the reaction: Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. MOGAT2 has a preference toward monoacylglycerols containing unsaturated fatty acids. MOGAT2 plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. And it plays a role in diet-induced obesity. The antibody can recognize all these 3 isoform of MOGAT2.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity.; Authors - Zhuzhen Zhang
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
pig	WB	Metabolites; Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease; Authors - Jianping Xu