MUTYH Polyklonaler Antikörper

MUTYH Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15237-1-AP

Synonyme

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 15237-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15237-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15237-1-AP bindet in WB,ELISA MUTYH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUTYH fusion protein Ag7359
• Vollständiger Name: mutY homolog (E. coli)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 65 kDa
• GenBank-Zugangsnummer: BC003178
• Gene symbol: MUTYH
• Gene ID (NCBI): 4595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.