SRC3/NCOA3 Polyklonaler Antikörper

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• KD/KO Validated

SRC3/NCOA3 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20032-1-AP

Synonyme

ACTR, AIB 1, AIB1, CBP interacting protein, KAT13B, NCoA 3, NCOA3, nuclear receptor coactivator 3, pCIP, RAC 3, RAC3, SRC 3, SRC3, SRC3/NCOA3, TNRC14, TNRC16, TRAM 1, TRAM1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20032-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20032-1-AP (NCOA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20032-1-AP

  Immunofluorescent analysis of HepG2 cells, using NCOA3 antibody 20032-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

20032-1-AP bindet in WB, IHC, IF, ChIP, ELISA SRC3/NCOA3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nuclear receptor coactivator 3
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NM_006534
• Gene symbol: NCOA3
• Gene ID (NCBI): 8202
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. The antibody can recognizes all the isoforms of NCOA3.

Publikationen

Species	Application	Title
human,mouse	ChIP	J Biol Chem; A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator; Authors - Xuan Zheng ; KD Validated
human	WB	J Biol Chem; A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator.; Authors - Xuan Zheng ; KD Validated
human	WB,IHC	Biomed Pharmacother; PMID: 32036219; Authors - Yu Chen ; KD Validated
human	IHC	Medicine (Baltimore); Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma; Authors - Jia Liu