Tested Applications
| Positive WB detected in | A431 cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18701-1-AP targets NUMB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | numb homolog (Drosophila) |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 66-72 kDa |
| GenBank Accession Number | BC068476 |
| Gene Symbol | NUMB |
| Gene ID (NCBI) | 8650 |
| RRID | AB_2154286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49757 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NUMB antibody 18701-1-AP | Download protocol |
| WB protocol for NUMB antibody 18701-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NUMB Polyclonal antibody (catalog no. 18701-1-AP) has 9 citations across journals including Cancer Research, Advanced Science, Cancer Letters, Neurobiology of Disease, Frontiers in Cell and Developmental Biology, Journal of Cellular and Molecular Medicine, Frontiers in Oncology, Tissue and Cell, and Oncology Letters. Research fields span cancer biology (lung, bladder, breast, liver, colon, and small cell cancers), cell polarity and trafficking, epithelial-mesenchymal transition, Notch signaling, and osteogenic differentiation, with applications mainly in Western blot and IHC.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) PP2A Promotes the Symmetric Division of MUC1-Dominant Cancer Stem-Like Cells in Small Cell Lung Cancer | ||
Cancer Lett Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer. | ||
Neurobiol Dis The polarity protein Par3 regulates APP trafficking and processing through the endocytic adaptor protein Numb.
| ||
Front Cell Dev Biol IFT20 Mediates the Transport of Cell Migration Regulators From the Trans-Golgi Network to the Plasma Membrane in Breast Cancer Cells. | ||
J Cell Mol Med Musashi2 predicts poor prognosis and invasion in hepatocellular carcinoma by driving epithelial-mesenchymal transition. |
