NUP85 Polyklonaler Antikörper

NUP85 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19370-1-AP

Synonyme

85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75, Nucleoporin Nup85, Nup75, NUP85

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse lung using 19370-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19370-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19370-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells using 19370-1-AP

  IF result (Nucleus stain) of anti-NUP85 (19370-1-AP; 1:50) hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, HeLa-Zellen, humanes Hirngewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

19370-1-AP bindet in WB, IHC, IF, ELISA NUP85 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP85 fusion protein Ag7086
• Vollständiger Name: nucleoporin 85kDa
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC000697
• Gene symbol: NUP85
• Gene ID (NCBI): 79902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB	Hum Mol Genet; Expanding the phenotype of NUP85 mutations beyond nephrotic syndrome to primary autosomal recessive microcephaly and Seckel syndrome spectrum disorders.; Authors - Ethiraj Ravindran
human	WB,IF	Front Microbiol; Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein; Authors - Yue-Huan Ling ; KD Validated
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	WB	Front Neurol; Case report: Compound heterozygous NUP85 variants cause autosomal recessive primary microcephaly; Authors - Ethiraj Ravindran