OAS2 Polyklonaler Antikörper

OAS2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14214-1-AP

Synonyme

(2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 14214-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14214-1-AP (OAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14214-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14214-1-AP (OAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14214-1-AP (OAS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14214-1-AP (OAS2 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14214-1-AP bindet in WB,ELISA OAS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OAS2 fusion protein Ag5436
• Vollständiger Name: 2'-5'-oligoadenylate synthetase 2, 69/71kDa
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 66-71 kDa
• GenBank-Zugangsnummer: BC049215
• Gene symbol: OAS2
• Gene ID (NCBI): 4939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS/p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody recognizes isoform p71 and isoform p69 of OAS2.