P21 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

P21 Monoklonal Antikörper für ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Affe und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

CloneNo.

5F2F3

Rezensionen (2)

Kat-Nr. : 60214-1-Ig

Synonyme

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

---

• 

  WB analysis of MCF-7 using 60214-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60214-1-Ig (P21 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60214-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60214-1-Ig (P21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 29 publications below

Produktinformation

60214-1-Ig bindet in WB, ELISA P21 und zeigt Reaktivität mit Affe

• Getestete Reaktivität: Affe
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: P21 fusion protein Ag0308
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Berechnetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000312
• Gene symbol: p21
• Gene ID (NCBI): 1026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	WB	Biomed Pharmacother; MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3.; Authors - Xiangdong Bai
human	WB	J Pathol; NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.; Authors - Rong-Zong Liu ; KD Validated
human	WB	Virulence; Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.; Authors - Wei Jie
		Int J Biol Sci; Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.; Authors - Miao Bai
	WB	Aging (Albany NY); Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation.; Authors - Rika Imai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Ying (Verified Customer) (02-02-2021)

it is very good antibody.

• Applications: Western Blot

FH

Chunxia (Verified Customer) (02-02-2021)

Tissue culture

• Applications: Western Blot, Cell culture