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P21 Monoklonaler Antikörper
P21 Monoklonal Antikörper für ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
Affe und mehr (1)
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
CloneNo.
5F2F3
Kat-Nr. : 60214-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 28 publications below |
Produktinformation
60214-1-Ig bindet in WB, ELISA P21 und zeigt Reaktivität mit Affe
| Getestete Reaktivität | Affe |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | P21 fusion protein Ag0308 |
| Vollständiger Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Berechnetes Molekulargewicht | 21 kDa |
| GenBank-Zugangsnummer | BC000312 |
| Gene symbol | CDKN1A |
| Gene ID (NCBI) | 1026 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for P21 antibody 60214-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3. | ||
J Pathol NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.
| ||
Virulence Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions. | ||
Aging (Albany NY) Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation. | ||
J Biol Chem RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53. | ||
Front Pharmacol Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ying (Verified Customer) (02-02-2021) | it is very good antibody.
|
FH Chunxia (Verified Customer) (02-02-2021) | Tissue culture
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