P4HA1 Recombinant monoclonal antibody

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• P4HA1 Recombinant monoclonal antibody (86825-1-RR)

P4HA1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86825-1-RR

Clone No.251813G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1

• 

  WB analysis using 86825-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86825-1-RR (P4HA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 86825-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86825-1-RR (P4HA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, PC-3 cells, HEK-293 cells, MCF-7 cells, ACHN cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86825-1-RR targets P4HA1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3292

  Product name: Recombinant human P4HA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 238-534 aa of BC034998

  Sequence: HQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

• Full Name: prolyl 4-hydroxylase, alpha polypeptide I
• Calculated Molecular Weight: 534 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC034998
• Gene Symbol: P4HA1
• Gene ID (NCBI): 5033
• RRID: AB_3745145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13674
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P4HA1 (Prolyl 4-hydroxylase subunit alpha-1) is also known as P4HA and belongs to the P4HA family, which plays a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exist as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has some isoforms with the MW 61-63 kDa and 57-59 kDa.