PDK1 Polyklonaler Antikörper

PDK1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 10026-1-AP

Synonyme

PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 10026-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10026-1-AP (PDK1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10026-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10026-1-AP (PDK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10026-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10026-1-AP (PDK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10026-1-AP

  IP Result of anti-PDK1 (IP:10026-1-AP, 6ug; Detection:10026-1-AP 1:1000) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human small intestine using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10026-1-AP (PDK1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10026-1-AP (PDK1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Dünndarmgewebe, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Kolongewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IHC: See 3 publications below

Produktinformation

10026-1-AP bindet in WB, IP, IHC, ELISA PDK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDK1 fusion protein Ag0025
• Vollständiger Name: pyruvate dehydrogenase kinase, isozyme 1
• Berechnetes Molekulargewicht: 436 aa, 49 kDa
• Beobachtetes Molekulargewicht: 46-55 kDa
• GenBank-Zugangsnummer: BC039158
• Gene symbol: PDK1
• Gene ID (NCBI): 5163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is speicfic to PDK1.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.; Authors - Shaoxun Xiang
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
mouse	WB	Front Endocrinol (Lausanne); HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway.; Authors - Wen-Ning Xu
		Cells; Prox1 Suppresses the Proliferation of Breast Cancer Cells via Direct Inhibition of c-Myc Gene Expression; Authors - Artemis Michail
rat	WB	J Cell Mol Med; PMID: 31945804; Authors - Chenyang Chen
human	IHC	Front Oncol; Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma; Authors - Yu-Hsuan Kuo