PEX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PEX3 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 10946-1-AP

Synonyme

Peroxin 3, Peroxin-3, PEX

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• 

  WB analysis of HeLa using 10946-1-AP

  WB result of PEX3 antibody (10946-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX3 transfected HeLa cells.

• 

  IF Staining of mouse brain using 10946-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PEX3 antibody (10946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 10946-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PEX3 antibody (10946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF-P: Mausnierengewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

10946-1-AP bindet in WB, IF-P, ELISA PEX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, arabidopsis, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEX3 fusion protein Ag1384
• Vollständiger Name: peroxisomal biogenesis factor 3
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC014551
• Gene symbol: PEX3
• Gene ID (NCBI): 8504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 3, also known as PEX3 (peroxisomal biogenesis factor 3) or TRG18, is a 373 amino acid multi-pass membrane protein that anchored in the peroxisomal membrane and essential for peroxisome biogenesis and integrity. Additionally, Peroxin 3 acts as a docking factor for Peroxin 19 and is required for the import of peroxisomal proteins. Defects in the gene encoding Peroxin 3 are the cause of peroxisome biogenesis disorder complementation group 12 (PBD-CG12) and Zellweger syndrome (ZwS), both of which arise from a failure of peroxisomal protein import.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter
human	WB	Cell Death Differ; Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.; Authors - Weiwei Cui
mouse	WB	Kidney360; Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism.; Authors - Takeshi Terabayashi
mouse	WB	Methods Mol Biol; Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.; Authors - Miriam J Schönenberger
mouse	WB	Biochim Biophys Acta; Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.; Authors - Kovacs Werner J WJ
Arabidopsis	WB	J Integr Plant Biol; PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants.; Authors - Sarah E Burkhart

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (07-14-2022)

BSA block in TBS

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293

PEX3 Antibody Western Blot validation (1:500 dilution) in HEK293 (Cat no:10946-1-AP)