PI3 Kinase p110 Alpha Polyclonal antibody

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• PI3 Kinase p110 Alpha Polyclonal antibody (27921-1-AP)

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 27921-1-AP Copied!

Publications (35)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PI3K p110(alpha), PIK3CA, EC:2.7.1.137, EC:2.7.11.1, p110 alpha

• 

  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 27921-1-AP

  IP result of anti-PI3 Kinase p110 Alpha (IP:27921-1-AP, 4ug; Detection:27921-1-AP 1:1000) with mouse brain tissue lysate 1680 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 27921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 27921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27921-1-AP targets PI3 Kinase p110 Alpha in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27539

  Product name: Recombinant human PIK3CA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 345-550 aa of BC113601

  Sequence: NVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDF

• Full Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Calculated Molecular Weight: 1068 aa, 124 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC113601
• Gene Symbol: PIK3CA
• Gene ID (NCBI): 5290
• RRID: AB_2918135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42336
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publications

What published studies show

The PI3 Kinase p110 Alpha Polyclonal antibody (Cat# 27921-1-AP) has accumulated 35 citations across journals including Nature Communications, Advanced Science, eLife, Cell Reports, Journal of Advanced Research, and Journal of Ethnopharmacology, among others. Research fields span oncology (breast, lung, ovarian, and colorectal cancers), PI3K/AKT signaling pathway modulation, metabolic disorders including obesity and insulin resistance, inflammation and fibrosis, cardiovascular disease, neuroinflammation, and natural product pharmacology. The antibody is primarily used for Western blot and co-immunoprecipitation applications.

Automatically generated from the 35 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; Microbiota-indole-3-propionic acid-heart axis mediates the protection of leflunomide against αPD1-induced cardiotoxicity in mice; Authors - Rong Huang
rat	WB,CoIP	J Adv Res; PTPN22 as a therapeutic target in intervertebral disc degeneration: Modulating mitophagy and pyroptosis through the PI3K/AKT/mTOR axis; Authors - Haibo Liang
	WB	Adv Sci (Weinh); Bufalin Inhibits the PI3K/AKT Pathway by Targeting GTF3C4 to Impede Breast Cancer Progression; Authors - Siyu Guo
mouse	WB	Phytomedicine; Icariside II modulates pulmonary fibrosis via PI3K/Akt/β-catenin pathway inhibition of M2 macrophage program; Authors - Lingling Deng
rat	WB	Adv Healthc Mater; Transdermal Delivery of Icariin via Microneedle Patches Attenuates Phthalate-Induced Testicular Injury.; Authors - Chunyan Fang
mouse	WB	Int J Pharm X; Topical transdermal administration of lenalidomide nanosuspensions-based hydrogels against melanoma: In vitro and in vivo studies; Authors - Mengdi Zhang