PLK4 Polyklonaler Antikörper

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• KD/KO Validated

PLK4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 12952-1-AP

Synonyme

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

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Galerie der Validierungsdaten

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  WB analysis of A549 cells using 12952-1-AP

  WB result of PLK4 antibody (12952-1-AP, 1:2000) with si-control and si-PLK4 transfected A549 cells.

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  WB analysis using 12952-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12952-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12952-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, A549-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Lungenkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 10 publications below
• IF: See 6 publications below

Produktinformation

12952-1-AP bindet in WB, IHC, IF, ELISA PLK4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLK4 fusion protein Ag3605
• Vollständiger Name: polo-like kinase 4 (Drosophila)
• Berechnetes Molekulargewicht: 970 aa, 109 kDa
• Beobachtetes Molekulargewicht: 105-112 kDa
• GenBank-Zugangsnummer: BC036023
• Gene symbol: PLK4
• Gene ID (NCBI): 10733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLK4(Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse, human	WB, IHC	Nat Commun; Loss of KLF14 triggers centrosome amplification and tumorigenesis.; Authors - Guangjian Fan ; KO Validated
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human	WB,IHC	Cell Death Dis; Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway.; Authors - Xiangdong Tian ; KD Validated
human	WB	Cell Death Dis; NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.; Authors - Min Li
human	WB	Mol Cancer Ther; Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo.; Authors - Ke Liu