PPAR Gamma Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PPAR Gamma Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

4E12F10

Kat-Nr. : 60127-1-Ig

Synonyme

CIMT1, NR1C3, PPAR gamma, PPARG1

---

Galerie der Validierungsdaten

• 

  WB analysis using 60127-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 60127-1-Ig

  WB result of PPAR Gamma antibody (60127-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAR Gamma transfected HL-60 cells.

• 

  IHC staining of human prostate cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 60127-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon using 60127-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of K-562 using 60127-1-Ig

  1X10^6 K-562 cells were stained with 0.20ug PPAR gamma antibody (60127-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Plazenta-Gewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Kolongewebe
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

60127-1-Ig bindet in WB, IHC, IF, FC, ELISA PPAR Gamma und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPAR Gamma fusion protein Ag10005
• Vollständiger Name: peroxisome proliferator-activated receptor gamma
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC006811
• Gene symbol: PPARG
• Gene ID (NCBI): 5468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publikationen

Species	Application	Title
mouse	WB	J Physiol Biochem; miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2.; Authors - Chen Chen
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
mouse	IHC	J Immunol Res; Identification of Adipogenesis Subgroups and Immune Infiltration Characteristics in Diabetic Peripheral Neuropathy; Authors - Yumin Lin
sheep	WB	Int J Mol Sci; The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432; Authors - Meilin Jin