PSEN1 Polyklonaler Antikörper

PSEN1 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20434-1-AP

Synonyme

AD3, FAD, presenilin 1, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182

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  IHC staining of human gliomas using 20434-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20434-1-AP (PSEN1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 20434-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20434-1-AP (PSEN1 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 20434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20434-1-AP (PSEN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20434-1-AP (PSEN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 20434-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSEN1 antibody (20434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 20434-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSEN1 antibody (20434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 20434-1-AP

  Immunofluorescent analysis of HEK-293 cells using 20434-1-AP (PSEN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Maushirngewebe, HEK-293-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

20434-1-AP bindet in IF, IHC, ELISA PSEN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSEN1 fusion protein Ag14135
• Vollständiger Name: presenilin 1
• Berechnetes Molekulargewicht: 467 aa, 53 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC011729
• Gene symbol: PSEN1
• Gene ID (NCBI): 5663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a ~30-kDa N-terminal fragment (NTF) and a ~20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody can recognize all the isoforms except isoform 4 (21 kDa).

Publikationen

Species	Application	Title
mouse	IF	Front Aging Neurosci; LongShengZhi Capsule Attenuates Alzheimer-Like Pathology in APP/PS1 Double Transgenic Mice by Reducing Neuronal Oxidative Stress and Inflammation.; Authors - Zequn Yin