Nectin 2 Polyklonaler Antikörper

Nectin 2 Polyklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10100-2-AP

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 10100-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10100-2-AP (Nectin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 10100-2-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10100-2-AP (Nectin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10100-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10100-2-AP (Nectin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 10100-2-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Nectin 2 antibody (10100-2-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, 3T3-L1-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

10100-2-AP bindet in WB, IF, FC,ELISA Nectin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nectin 2 fusion protein Ag0149
• Vollständiger Name: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Berechnetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC003091
• Gene symbol: PVRL2
• Gene ID (NCBI): 5819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2, synonyms: HVEB, PRR2, CD112, PVRR2) is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111 , CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V, C, C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. This interaction is mediated by a sequence located to the C terminal ends of both isoforms of CD112 (A/ExYV) and the PDZ domain of afadin. This sequence is also found in CD112 and PRR3 and represents a specific consensus in the family, which is also named nectin family. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses

Publikationen

Species	Application	Title
monkey	WB,IF	Eur J Cell Biol; Membrane palmitoylated proteins regulate trafficking and processing of nectins.; Authors - Dudak Amanda A